Main Scientific Question of the Project :
This project aims to explore the factors contributing to the high fidelity of DNA polymerases in KOd1, a hyperthermophilic archaeon known for its robust DNA replication mechanisms. By characterising the specific properties and interactions of these polymerases, we seek to understand the molecular basis for their remarkable accuracy.
Project Short Description:
This project aims to explore the factors contributing to the high fidelity of DNA polymerases in KOd1, a hyperthermophilic archaeon known for its robust DNA replication mechanisms. By characterising the specific properties and interactions of these polymerases, we seek to understand the molecular basis for their remarkable accuracy. This research has implications for biotechnology applications, where high-fidelity DNA replication is crucial, and could provide insights into the evolution of DNA replication machinery in extreme environments. The project involves a combination of biochemical assays, structural analysis, and comparative genomics to uncover the underlying mechanisms of polymerase fidelity.